4-PEG介导的花生疮痂病菌遗传转化.docx

参考文献：2002-EnginCCringagenetictransformationsystemforCo1.1.ctotrichumacutatun.thecausa1.fungusof1.imeanthracnoseandpostb1.oonfruitdropofcitrus花生疮痂病菌的遗传转化目的：建立花生疮摭病菌的遗传转化体系，为后续基因功能的验证莫定基础方法：1 .在非选择性培养基上对阳性标记进行筛选（抗生素筛选）2 .原生旗体制备3原生质体再生（培养方法、培养基筛选）讨论：1. Materia1.sandmethods1.1. Funga1.iso1.ates,cu1.tura1.conditionsandsensitivitytests病原菌来源表格A1.1.iso1.ateswerecu1.turedfromsurfacesteri1.ized1.eavesofinfectedp1.antsandweresubsequent1.ysing1.e-sporeiso1.ated.Forprotop1.astpreparationandinocu1.ationexperiments,unga1.cu1.turesweremaintainedroutine1.yonpotatodextroseagar.Thestabi1.ityoftransformantswasassessedbytransferringsu1.fony1.urea-resistanttransformantsonceaweekonanon-se1.ectivemediumforfivesequentia1.weeks,fo1.1.owedbytransferbacktothemediumcontaining50gn1.su1.fony1.urea.（磺院W）通过横解限类药物ThesensitivityofS.atoVariOUScompoundswasdeterminedtoidentifyapositivemarkerfortransformationse1.ection.