PGRs对愈伤成分种类及其含量的影响.docx

PCRS对*仿油»3共有效成分的影看Effectofp1.antsg11>thregu1.atorsandIigh1.onthv(>routhb1.oyntbeiofadhecompoundsInDictammisdasycarpusTUrCzOdIgcu1.turesO1.KKuniMic(*1MI).fraine1.1.onc(MM),IimoninIrtMHt>Dk1.ammnv(StNt)目的，1. MRf"含仿生氏HUZH资态现成的影响,分切找公优配方.2. PGR，"金仿中成分种类24大分Ia侑影响,分别拉世优配方.3. 光照时影侪形.6促成.生长和活性成分/Ift的比咱内M(Mft>*ftW为X0tEfSM.5t三j1.,KT工OnISNAA1.生火力It饰生长、海gt成分ISUM至1.*2.4-D.IA,IBA.NAA.-NOA.TIBA,4PAK1.t1.taiOSKT口.O生长率<7S1.i.何处阀6肢法段用2次)何料用养权连相融代不切)3次？取样渊定,JtmMMBtti宪饬图片颜色和木炮)卜M.DW.Gi,或分种突和台及Iai臼的，明确生KA对患折厦胞、幽色、卞长、N分畀黄印含M的影喻.工分*JI憧体生长、MrWt.虚分用!SUM*jR>KT.BAKZTsTOZ.HCPPUIADPUOftM*054衿分裂点,20me,1.24D(6蛆试验,施处理，收.I%"(U2次)H朴培脩携煌纨维代3次？取样恻定.I6hftffiSHMMh念伤图片颜色和盛地)、2DW,Gk成分种炎和JThtKIi目的I月时分裂卡对必仿旗地.闻色.'1.K成分加类和合以算彰响.3.W*M>*力率hMSQ5分裂去包0WW1.2.41)MdGOme1.ZADKINAAsinIZONAA2.4DaIftAQWfNAA粕2.4D压少=>不加.111NAAffift/方案2，MS*05KT2Qmg1.,2.4-1)*2Xm'1.14-H2.<)NAAIBAJAA)2.011y1.NAA"BA，IAA1.上面己有结果34TWT*分皿10多个HE方优化生长M0州"优化、生长、代次机=仿生长、Na«.分畲量的M设正竞鼠.6尤服.那0*1. PG以段其筑介调件有牧成分种类及K含气2比皎念仍不利状态(雀色和依地或分种美及含裕的关肩3. W价2.4D的作用：促避免物生长郎加制成分片受和含*4. 获用小a分或蛆合多个橇分的0优用方5. I早价光膜的作用，九%不利了史仿生长利金伤变色.利T4.方生成注，1仿色译和履*，唐的色城“色索小长股的负仿产上生物HBa1.rkyC1.hmEgEIEO叽e11MqggruHk>w取wihr*amAnMiMrmvwmkM/N贝色.吩砰的'IK快妁产牛，1批找少(thePa1.Cye1.1.ow.EoCrfrui1.c,growingca1.1.u>BUa1.1.yhau1.owc<ub<1.i1.yofa1.ka1.oidSyntbe¼M)2.ABi任影伤支坡叶地/仆成j促进或分种丈及含植包卯M生匕.X2.4-DI优避金伪形成和生KWtt11fe.但和制成分士类利含小Effectofp1.antsrowthregu1.atorsand1.ighton(hegrowthImdbiosynthesiso(activecompoundsinDic1.aniiiusdasycarpusTurcz.ca1.1.uscu1.turesQuindineu1.U1.dvAbstractIn(hepresentedwork,thecf1.ec1.ofp1.antgrowthrcu1.atoc*.inc1.uding05mg,1.Ievdof7auxins(2.4-1).IAA.IBA.NAA.B-NOA.TIBA.4-CPA)and2.0Ina1.k、U1.of6Cyiokintns(K)NBAP.ZEA、TD7、4-CPP1.KU-DPU).wasexaminedf<x,Ihcirincncconbio11uvigwuthandtheacmub1.inofmciii11j11.y-rc1.cvin1.biictivecnmpcHincis(<bicu11one,Fnuine1.1.one,1.im<>ninax1.DiaamninOinca1.1.uscu1.turesofDkumwusdasycarpus(Rutaeene).成分A类和含量是可变的Theresu1.tmUwedI1.ia1.(bev;IneIyadPrOduC1.ionMbiuc(iveCernpCX1.fXbinca1.1.uscu1.tureofD.daxycatpuscanbem<x!uhtc!.dependingonthecombinationofp1.antgrowthrcgu1.acor分裳素中优AmOn8IheCyIOkiIIinSe.vxi11ents.I1.urIgXimUmgn>½1.h(bW.DW.Gi)WegobtainCdKH0.5m。1.KINinCombirnitinnwith2.0m。1.2.4D.Hnwxrvcnthehighestcontent(#>ofDiciamniiK(XKmoreimx>rtantconstituent,werercco11fcdintheca1.1.uscu1.turedonMSso1.idmediumsupp1.ementedWiIh05mg1.KINincombinationwith2.0IAA.生长*ttInthegroupofauxins,theCombtnaikNiof0.5ng'1.XXXand2.(hng,1.YYYPrOVidCdthef;IjiteMcu1.tureKroWth(Gi=).Thecn11ccnf11tfk>naMofactivePhtodKTniC工人inca1.1.uscu1.tureswassimi1.ar(otha<ofwi1.dptants(含，b仿生长和成分与生长素负相关.与分裳素正相关Siaiisuca1.ana1.ysisofthere1.ationshipsbetweenca1.1.u%growthandI1.uracvumu1.a1.i(ofactivePhkXrhetnieak1.x>wedp<si1.iveCD11eh1.inuthecy1.nkininsandnegativeComHii1.innwithauxins.元*Oufresu1.tssuggestIhMIighiregu1.ateIifnOn1.nSax1.dicamnnbwsyiMbcsisandhcdiffere1.a!HnOatuscfca1.1.u%cu1.ture%.Quaii1.i1.a1.iveCHIg1.iUnof*waxert<t<edbyusingHP1.eana1.ysis.I1.wasShOWnthat1.ightandp1.antgrowthregu1.atorssignificant1.yinf1.uencedthebiosynthesisof1.imoninsundDicianininc.Tothebe乂ofthenHwrknow1.edge.UiuwtheIirMsm.1iCOmPreI1.enSieu(w1.CuaICVnUngIbei11cnccofPGR力onbic>ma«growthandhcMxumuktfionof'bi<ctivcConIPn1.nIdinVi1.mC1.1.Ii、cuhurcsofD.daxyarpvs.Keywords:Bk>y1.ic%CaHUXDie1.annusdtaycarpus.IXiRs.Dur1.;IJ1.1.mneIntroduction-WWh介作用E八作用打JREB含Ir生生EHbMm至smiMm度.裳AWIHM合创WA廉11知的，优Jk«是白w11m*o什么白MMH介：Dicitimnux(Iax)WtrpuxTurcz(Rntacv)KcnCoftheimportantmedicina1.p1.antsax1.nativetoEaaAsia.Tbcrootharkofthep1.antsicd1.edCWTerdMamniinIhCChinesePharmacopoeia,v*hichisu«dinChineseIraditiona1.mcdmefortheIret1.nCIHofjaundice,cough,rheumatism收KM1.WEC&indgchM现代ISW九Inmodempharmaco1.ogica1.studies,(hep1.anehasbeenreportedtoPIayvariousbio1.ogica1.ro1.esincancer,inf1.ammak>n.andobesityinmanydifferedce1.1.Jypcsandtiuc4uxitobeM1.iP1.皿*罐Zrega1.tion.vucu1.arn7Jing.muhctcria1.n(ifungu1.jCtiVie延W*分TheCnCenWUSthcmpcuticimpotunceandbi<>k>g>c1.ctivitk).Qf1.dtwrpui(I1.KUnIyduecoConUh1.grcmMnotIn1.ofIimonmsanda1.ka1.oids.Among(bcm.1.imonoid,dictamni.Obacunoncadfraxinc1.1.o<arcthenoMdcmonMncdrcrescnuvc1i.原料主SBHI于根破坏性H<wcve,QbCybi.IiVeComJXH1.1.Kkof7<iaxycarpaarcmain1.yCnncen1.nitedinthehrkan<ir<×Mofthep1.antandContimiouvJydejn>ctivchins1.ofIheWikJp1.antresu1.tinginthepopu1.ationofWikJresourceincraising1.ydec1.inedordisappearedfromitsnatura1.habit,bKHnxonnc>dcd.代tfcftTheefbrcinvi1.rbnynthesisofbi<w(ivcCnmfXHHKkIhnxighp1.anttissuecu1.1.u11:techniquemayprovideunakc11utivcapproachforso1.vingthisprob1.em代技术研允JXIBiOSyfHhEs<secondarynabo1.itcsthroughp1.anttissuecu1.tureisaWd1.kmwnapproachadrecent1.y¼airvptvtedfurM1.rhnofarungeufbixc(ivecnM>umhfrndiffcmp1.antsviz.a1.ka1.oids.f1.avonoid(货Si类)附类、等等IiQ入引文fromt*ttT4Sp1.ants.IY；R、AXttJMt9(A1.tURPIMmw1.hregu1.akareone<>1.IiienDtiniptv1.aii1.factuu(fee1.ingce1.1.grow1.h.dIYervii1.ia(i<mandme1.1.tXTi1.eroduc1.i1.Mod!ica1.>nsofp1.yk>1.x>11nnecunpxt1.nocrwidepossibi1.itiesofimprovingce1.1.CUhUrCproductivitywithrespecttothcrupciica1.1.yrcMauCanMiIUcm、dU6*少相关生长节¾控生长和成分含工的液在¾U(看文”补上)己有奴多学开JR相美瑞九，某某人事允俺提育“,成分ME1.MMf1.1.nI川HnmbMe1.fUK1.a>11jo)fxi,<,iwrAVviMw4vj.CbeMBiryo1.dIrdu¼vyPtcm:BCg20102)J1.cictU1.GxnpiMiiiis.cUk>xki1.>aidantetikruh&xivy<(w11u1.ni1.fmwDkurm(isy<ar11RwxiCMfIMiTy.16"1.>SXrt.X*M1.M；：KxiiH.KimMKimHSM1.UeQGmAnW.d，S:AtIiatnMmMHitiyMditi1.ie*UrH÷tmt(day<>rptz.EMbockE.i1.krpvEEKIdc11Mid<dbyU1.nITanh11MVCnetnIWCeJMhmMnnKdX»U.r2t4T-411.3XC(M(BMhX1.QUtO1.itM)JnJIMnmN“bb1.MUvi<fo*nrtu1.ui1.fnmDkImimu*dM>wfu*pc1.fttIMmiV-YcCCJ.C.Km.S.H.Km.M.J.Vang.H.J.Ah>.MR.&Par.JH(2015).1.11cmn.Ccmponen1.ct010a1111ftadsCorte«.InhtrtsEacciInducedCdkiumardGAVP1.ae匕8MFKKCRE8Sne<X)P卅即刈GNCC-M3T3UCeW%g*E纯.2212822136.f1.ttK1.W-31.*PNnxWne1.ka1.im<k>1.rHiitam»1exoc1.ktatd><tfdk1.Mur.a11uxHaixtiwox<xwmiimwHr11×*KiiiDirtxntusdny<afp<1Uv.fK<c>4¼JI3XHG)MPeftCCfreajn<fi1.cofSfMomy<e<cHZitdcdbydk<rni(K.fdftttIMMS*5*Prt*MjMINUHCvKQhNmik*Zbyhi"erxtmg:Uudctrc<uktr<)tt)*fcCkr1.exfnnabtn“»evea«&<iwcc«iipiM»1.»Ccv1.cDicUntti.pdf201WSiEsrwiMd，”rnii,;”>nnfIirentin1.dmnv<kCiNOMmcmh1miMKk<4H附5冏力泣IWrMkIttMUB*;-f-daituia<<xx*J<«11UiM1.it4>fwnndwpfim:ra>rp*xm»1JPn«»dw，fccKihtw11p11nr11wMan4E加“MVWndXE白IM1.BiH1.W%JS*Inpreviousreport,invircu1.turesofD,Ssyca/pu*wasestab1.ishedinM1.ertoPrOVk1.eanefficicmrcgenerauonPIOIOcoI(引用If几白IUftie的中文文”和自己的上一比攵套)白事*少It方面的研究However.contrarytoothermedicina1.p1.ants,theresearchconcerningtheinf1.uenceofp1.umgrowthreuho5CHMhCMiuctionofSeCandafymetabo1.itesarcratherscarceinvitroiiica!1.u%cu1.(ure%ofD.daxycarjnts.本文开JW¾研究内ThC建imofthepcr%cntc1.Xnrdwaxtoex1.imatetheinf1.uenceofdifferentcombinationsofp1.antgrowthreg1.u(ocsonbiomassPOWthandtheaccumu1.ationof1.imonoid*dic(amnine.obacu<HKandfruxinc1.1.o<c.themostdcnonsraic<1.rcscntativecompoundsinD.day<aruxca1.1.uscu1.Iutvs.Tothe3u1.tu>r'knnvkdge.thisisI1.icfir¼tsuchc>nfuhenMvestudyonbiomussgrowthUndtheuccmu1.aiionOfbkxactivccompounds<onthePhy1.ohBmCmeregu1.ationof1.imooid.diciamne.ObaCUnonCandfraxinci1.oncmciao1.ism>invitroinca1.1.uscu1.tures“D.diuyarpus.Materia1.sandmethods*ttJR始念AK方是MSaHUmg1.K20m矶,2*DP1.antmateria1.sThercviouUyCStabnShCdca1.1.usCUkUrCSofDiciannuidascafpuigrownonMSndiumcontaining0.5ng'1.kinetin(KIN).2.0mg1.D-Dichkmptienuxyaceticacid<2.4-D>and3.0%w/vsucrosewereusedasaMX1.nxofp1.antmacria1.andsubchuredat30daysinwnnk.J<分定是否AHESubcu1.ture(hrHmCH代三次？根>1一文/鳍票，EffectoPGRrnnca1.hisgrowthandhk>ynthc%i%ofhM>actieCCmPm1.nd芥In<xxcrtocva1.ii；uctheCfT(XKofauxim<>nca1.1.uxgr1.handthe4ccumu1.a(i<Hiofbio11c(iveCOmP(Xi1.XkinDietannw&dyearpu、ca1.1.uscu1.ture,theca1.1.uswereiubaicdonMSsdidmediumuppk11ven(edwith0.511g'1.KINinOfnbiiustxxnw1.1.2.<hng,1.1.eve1.erf2.4-1.)v1.1B.N.PM)A,TIB,4-(,P,respective1.y.The1.1.ucr<>wnonMSmediasupp1.cmcntc<!with(>.5mjj'1.KIN<m1.yi1.ho11no11e-frccwasconsideredascomrcMMimp1.cs.AiKiIogousIy.thecxpcrncnwasPCffDmWd10dctc11mtheeffectofcyiokimnsonca1.1.usgr<>u,1.handIheA.-cuinu1.a1.ionofbiuactiveCUmpoUnihuDiciannivicinycarpuica1.1.ucu1.tures.I1.ieca1.1.usWCEincuhatc<1.OnMSso1.idmediumUippicmcntcd0.5mg1.1.eve1.OfK1.N、BAP、力1.A、TDZ.4-CPP1.U1.3-DPUin<x>mbi½ionwt2.0ng,1.2.4-D.respective1.y.Theca1.1.usgrownonMSmediasup1.encdwhh2.0mg,24-Don1.yandhormone-freewereconsideredascontro1.%anp1.es.A1.1.experimentsurrcconductedusingMSso1.idmediumOOmaining3.0%w/vscrscand0.7%My邮ar.pHwas刈Ift1.Cdto5.8prior10autoc1.avinga1.I2X?tbf30mn.theIbCrmMyunstab1.eIKiRs<1.T1.BA,Zb1.,I)Z.4Xr,PU,B-DPU1.wereuddedtoUiePCVV)DU3yHU1.OCia、Nmediumbys<cri1.cfi1.trationthrough0.22Mmsyringet1.)wr¼,TherestQfIhCPGRSused“ereco-3U(oc1.avcdwithbasicmediumingedicn(.Ca1.1.uxcu1.turesvenan1.axx1.a1.25±2'CU1.MkTa16，'8h(Iigh1.cktfk)phMuperic>d(元强«K±8Mmo1.m:¾,.PhiIipsIW35WngrCSeCMk11ps.Eachca1.1.ustissue(rownfor30d)of0.2gfreshweight(0.04gDW)wasinitia1.incutxcdonI-Jnicyeff1.ak.v*ith30rep1.icas(6JK)per(rcatncntaid2次Andcchca1.1.usIkWCwasfttt-*<<hnMi*jkjj11iM11nnni11x*J11w<recvrefu1.1.>wn*e<JrnwnZ<utojrvwwdmJgktwfnn卬Eo1.i¢hth/h*dHedMIhePMfttufAikUiruIiiet1.*mef“妙bk<xo1.c*e11tbvi1.h¼Afjrci1.>rIm»bc.*11e3Ur½x*I1.aaitnfrrvdnnPrrAFZJhEirMmh>i1.Jne1.thewvis”Utrmir*MEJo11jcp»nd1.>1.RftttIrvfdir1./i»crcJwd1.ih?Iroi*wc<rvcwk01.«1H×CInu1.vc»1miIUf1.ttcM11WZAwdrtrTTairMtKXicf1.Nirdryu<iMibcukurcda1.34)daysinter,ak(几代，).Ca1.1.usIfghIwciRh;Indjryweighc、;ihi;NCdWHJHermrcrespective1.y.Gnnvthindex(Gi)wasca1.cu1.atedasIbCCkSCribOdby*”(引文)andtheequationWaSastb1.k>wnsGrowthindex*(WeiShIX-Weigh(.a>IWetgh<aa.Cai1.iwen*drieda60X?for48t.优化个阻力In(henextpanofthestudy,(heca1.1.uswwinocu1.atedonMS<x>11biutionuithdicrcnCemeVnuauonofauxinandcytokininptovidcdIhcbcMIcSUIginICtTn4ofcontent.EffectOr1.iKhtonCaH1.IXgm11handhi<xnthc×ixofactiveccimpnunc1.xTbcoi1.Iuswaxincuburte<iunderIhcdar.Can1.in1.1.aK1.ightand1.htpcr><×1.ofI6h,daywith1.ightsintensityof88+8UmO1.m2s,at25±2'C.Ca1.1.usfreshweightanddryWCigh1.WCreeva1.uatedacvrditoabovedescribedmethods.QtiantitMtiveJ1.nn1.y6f(H,tirCCE1.MX1.IHR91.1.tBX*ExtractsPrePUnHionundquaniita1.ivcHP1.Cana1.ysesofactiveComPm1.1.XJswerecffrncd.accordingtothePnXxdUrEdCScfibCdprevious1.y9t己*fcfc*1耳全的*Ixrthequami1.ivcana1.yxixofhi(urtiwSmpoMIXiS,1.OgofCadIPaWdercdO1.1.1.UAan<1.ftttIWM91-N1.VS11drt*r11n>1.r<H1.H<*i.dmnc.dMv>neMKJtr*mtUra<Mt1.意方法411RPJiP1.C对上测定门弊皮中3成分的公0一，Mtei.FdfWik1.p1.antsmutcri1.Waf1.11tmixedwith25m1.chann1.water(75:25,vv)andcxt11c1.e<i1.wnIimcvbybeat-ref1.uxingfor2h.Tbcextractionso1.utionsWCrCcombinedundfi1.tratedIhmygh0.22mnc11br3ie.AtIerrecoveringeIiiaxiu1.IheretKIUaIaque<xsWa、extractedIwoIinietbyIOin1.CH2CI2.1.,hetheCH2C12e.vtrac1.w=、ca3pora1.edtodrynessuungru1.an,evatrakwa1.amuximumtemperatureof451?IjHiI1.ThCcracsWerCdko卜CdviihIOm1.methano1.beforeHP1.Cana)ysikSa11katu1.ysi%waspcrft*medonaKronuisi1.100CISe4uxu(250nn×4.6mm.511)arx1.injectionvo1.umewa201.persamp1.eThemobi1.ePhacwnmdhan<>1.:H20(60:40.vv)a1.af1.owra1.eof1.Om1.min.Thedetectionwave1.engthandCdwnnICmPCm1.iHCWaSa236nmand35C.fCsp,hve1.y.A1.1.referenceMandarihwerePUrChaydfmmE1.Iiona1.inMituiefbrIheSmm1.CfPturnwEg1.adb)ogica1.ProdUe(.Thecocntra<>onsofxx.In>am1.csWCredecmincdbytheexterna1.Mandard11etMxJ.I>atanna1.)sA1.1.experimenta1.dataWCrCStatiMicaIIyaa1.yrodbyonewayana1.ysisofvariance(ANOVA)usingthePrV1.teCICd1.cast-MgncxHdi11cvncc(1.SD>test<P<0,05).andWCrCpresentedbyascragc土standarden>r<Sfc).Resu1.tsanddksionEmoau.hksonca1.1.ugrowthandbioyntH,s1.ofactivecompound、，INUtHYTheeffectofdiI1.erentauxinsonIhebi。Ina"gr<>uthanddieb<<xxr(nam1.en(inDidannUS<nrrQ>vca1.1.uscu1.tureswen:pre5tcdinFig.I.Cu1.1.usgrowthandtheconcentrationofbioactivccompoundsprovedtobeMrongiyaffectedbythetype4amountofIbCPGRsIIPP1.ied.AmongthephyichonnoiKtested,thehighestdumninca1.ka1.oidCon1.Cn1.waxacx:¼vdOnMSwi1.dmediumcmbuatic>n0.5ng<'1.K!Nad1.0mg<'1.1AA.加入某J*分不俺MA什么但俺整什么Theadditionof2Omg1.auxinshadnosignificanteffectonca1.1.usgroshbutSignif1.candyincrvcdtheaccmu)utionof*content.The7nntn1.andvyn1.he1.icauxinscommon1.yIIvdinVitm、showedconMt1.crab1.cCffE<>nbiomas、growthandtheaccumu1.ationofRhiactivecompoundsinDkMfinutdaSVCa，PUgca1.1.uscu1.tures产IwS和含囊受It料类和IMt的解，TheypcMdproductionofactiveCOmPOwkiSWCrCdirect1.yU1.I1.vCdbythetypeOfPhytohnrn1.nnCucdandiCxmcrntratinn(没做,衣履)inIhegrowthmedium.hwasdcmiincdthucauxins(2.4-D)addedtoMSmodiuaintheconcentrationof0.5mg1.genera1.1.yHthibi1.edIhcpn>ductM>11of.M比，1.ftT*auxins仿生长AcomparisonOftheGiva1.uesf11rhiom%xcsgrown<>nmediasupp1.ementedwith2.0mg.,1.QUXi1.Hshowedthathe(cstcdcompoundsMimu1.atcdthegrowthofIheca1.1.us.exceptforIAA.IBA.andNAA.优的Inh>serte¼ofcxpcrincu.(hefas1.e1.岁IM1.h(Gii力ax1.Ihehihes1.p111.uctiunc(*r11jgdrywci*hwereachievedontheMS11xxiiumMpp1.cncntcdwith0.5mg.'1.TIB?andO.5mg.'1.KIN.f1.11MAWMAACeSdingto<rGbHX机,ca31.u%withChrkye1.1.owGrgreenco!<r.*xnpc1.qiHi1.ityands1.(>w-mwthratecanKynthbiymon;a1.ka1.oids,whi1.ethePakyc1.k>w,moremgi1.c.fas-wingCaMUhusua1.1.yhasa1.owcapabi1.ityofa1.ka1.oidynt1.sis.especia1.1.yforttrtt.Tab1.eITheCffeVtof2.0mg1.sxin%COn1.bina1.ienwith0.511,1.KT<nIhcg11>wthandarumu1.aicnofCXucunonc.Fniiinc1.koe.1.1.moaIn3Dicwnniiicinca1.1.u>cu1.ture*AiIXinSFmh*dM<WeS240C<Mt(ib“<1.Wec411p<>Md©什DW>Cn1.1.mnt(>WCUIH<UFruxMvbKKI1.ic1.UiiinifMI2.4-D2±<oIO.2±)3o.2±.<02±2±(0IU2±0WWc>*>u,trp*e2IAAj'3IBA4NAADftrisv1.k>u.t*uf1.5ti-NOAI八TI1.八7MPAEffectofcytokininsonca1.1.usgrowthandbiosjnthcs1.sOractivecompoundsInccm(rustwiththeabovedescribedexperiments,effectofsixnatura1.orsyntheticc>,tokinninonca1.1.usgrowthaxibiosynthesisofacivcCofnPOUndSWCrCPeffbrnMx1.Aintheeaseofs1.dicitwaxohwvc1.thatcyinkininsNigniHGIm1.yinf1.uencedca1.1.usNmWIhandrodcioninca1.1.uscu1.tures.ThCCbsCrVCdcff<xisdcpcxJcdonthetypeofPGRsused,aswe1.1.asitCanCCIUraIiOnInthegrowthndium.IbwindetemuedUustexeep<(ZEAatx1.DPU,c>,Iokiniiua1.0.5ng,1.significant1.ystimu1.atedthercictinnof*andIntheseCxpcrimcnw1.conditions,aSignifkantincreaseofthecontentoftheinvestigatedMxondaryInC1.abO1.iIC4mca1.1.uswasa1.sonoted.OnIbCotherhand.IbCMSmediasu1.cncncdwith0.5m,1.ofthe%c1.ectc<icyu>kinininhibitedthe;