ISO TS 04971-2023.docx

TECHNICA1.SPECIFICATIONISO/TS4971editionFirst2O23-O6Nanotechno1.ogiesPerformanceeva1.uationofnanosuspensionscontainingc1.aynanop1.atesforquorumquenchingNanotechno1.ogies-Eva1.uationdesperformancesdesnanosuspensionsdenanofeui1.1.etsd,argi1.epourIequorumquenching©ISO2023COPYRIGHTPROTECTEDDOCUMENT©ISO2023IUirhM*hedbdi1.iUedotherwiseupdhi.or啪UIBndttaeDmk<nroni(ncm11ni10tf1.*Mqn1.C6pW11opypMRHt1.onmaytheinternetoranintranet,withoutpriorwrittenpermission.PermissioncanberequestedfromeitherISOattheaddressbe1.oworISO'smemberbodyinthecountryoftherequester.f),WV>fifiHipB1.andonnet8CH-1214Vernier,GenevaPhone：M1.22749O1.11觥ftte:丽丽BQrgPub1.ishedinSwitzer1.andAnnexA(informative)Re1.ationshipbetweenc1.aynanop1.atecharacteristicsandantibacteria1.performanceA.1.Genera1.Natura1.1.yabundantc1.ays,suchasmontmori1.1.onite,ha1.1.oysiteandirao!ine,genera1.1.yhavea1.ame1.1.arehajQiiboift!i1piha(roIiiiiuind1.ignnicp1.atcsandandnnoiibeofos1.HceAtt>fnectt)ftHtndt1.hnkfvouhih;efficacyforinhibitingbacteria1.activity.Ingenera1.,theefficacyisattributedtotheirhighspecificsurfacearea,surfacechargesandfinedispersioninaqueoussuspension,whichrendertheattractionA4s帕rHE照她也酣径由胡驮*HS网融出"ib般3福箱MM1.商速喻做OOthen招SKQ观部耿ypefort1.f1.kMi圈1.,w起<W玳靛d1.威?W即mens粉mayaffectthespacingen1.argementandsurfaceexposure.13Forexamp1.e,thespecificsurfaceareaofmontmori1.1.onite,exfo1.iatedmontmori1.1.onite,micaandexfo1.iatedmicaareabout750mzg,800mz/g,?用魂Ianand艇k阵岛眠他加出Ve1.y册出船nera1.,曲后整限ft三迩祖C1.ayS三*Bft%n保固屈板蛆geebacteria1.ce1.1.wa1.1.andstrengthenitsantiba<eria1.activity”A.3CationexchangecapacityThecationexchangecapacityvariesgreat1.ydependingonthec1.aysamp1.e.ThepresenceofsurfaceGHhrgWgonBioyhactomaesuEhdnQe'闻hwt1.yPngnISIkVI中IhCinHCShbi口8口弟Ihem圆户Siga1.into1.umps.Theantibacteria1.effectofthec1.aynanop1.ateexhibitsnonspecificforabroadrangeofgram-negativeandgram-positivebacteria1.species,butaffectingmostforthespecieswiththehighPirp1.iferationra1.eastheu1.timateresu1.tsbythephysica1.capturingandquorumquenchingproperties.A.4HydrodynamicsizeThehydrodynamicsizereferstothetota1.sizeofcoreandthesurfacemodifiedmo1.ecu1.esorso1.vationftdGdnano0ietesdvMut>iddtsuqjensionSBvcafiotItectouriSiipehratnttijtiyodynamicdne5erih(hdDuraqueoussuspensionconcentrationcan1.eadanaggregatedoragg1.omerateform,orthesurfaceionicchargescanbeso1.vatedbythepo1.arorganicsandwatermo1.ecu1.es网Theaggregationandso1.vationthe!tfei<ia1.effectofthec1.aynanop1.atesandthereforetheirantibacteria1.effect.Jcs,n邸Uraifb>WttRfi般fhfo曜刷川1。田祖袖碑鲂)hftwhf1.ftta1.届田刖快bf1.kftdpHobviouspropertyofpHdependency.Thephenomenonismain1.yaresu1.tofthec1.aynanop1.atecontaining1.argenumberofnew1.yexposedhydroxy1.functiona1.itiesonthesurface,whichorigina1.1.yexistedInisciriucingcMohatiiGwi1.htnitttectantfekijhaTjaAthfCtdMrim1.np1.dtfa1.andtbiH)fffiypeprge110fc1.aynanop1.atebyAnnexB(informative)Corre1.ationbetweenquorumquenchingabi1.ityandantibacteria1.performance1.gwiets11i>Hajfcas!ht<(mshb8cftivrtUxita(tethe(ijQMthnsingafiijpta1.rmbta11rt<ts(>ntth3tscta1.materia1.sprovidetheabi1.itytoquenchquorumsensingsigna1.mo1.ecu1.es.Thequorumquenchingabi1.ity(QQA)canbequantifiedandexpressedinunitsofmeq/100g.Mo1.ecu1.ar1.ywe1.1.-definedsurfactants三gWWStecationic,interaction.SinePrcsur三SI三taeHeristicth1.MP5theQQismeasuredandprobedbytheinteractionofdaynanop1.ateswithsurfactants.Inotherwords,theorigina1.surfactantCMCcandisappearduetothenanop1.ateinteraction.Thenoncova1.entbonding蛆糙窗MeM:哂UOniCSUrfaCtanC初除机能三而削匕端ngintc限眠酎龈郦眠,sur侑阳展渊也interactionwithedgehydroxy1.groupsforanionicsurfactant.曲威Wdifi?IWM&sbac*akur蒯赚Srt蝴腑Mbeco楸SSimU1.瑜姗艇ra巡视册OrcStf1.三1.颗nsingtheeftectivenessofnanop1.ateinteraGU4g-wUUmunicationmo1.ecu1.esofdifferentspecies.Theva1.ueofQQAbyca1.cu1.atingFormu1.a(1)tothecationicsurfactantHDTMA(CT11HaaN(CH3)3Br)and删Hf忠招幽喇&are醐三硼队那部Kate1.ynw明张确认哪砒tin脚/峥叫，ofionicchargesenab1.inganionicexchangereactioninanequiva1.entmannerforthenegative1.ycnargedc1.aynanop1.atewhenquenchingcationicsurfactant.Incontrast,thenotrionicsurfactant,octy1.pheno1.用fe三1.厢5的南9re(VPM舒6*胡卜也疑割胞自值%9QAobhkfe¾f(ard1.f1.etentsCMC.Hence,dependingonthechemica1.natureOfbacteria1.quorumsensingsigna1.s,thenanop)atestendtoexpressadifferentquorumquenchingabi1.itythatsubsequent1.yaffectingthebacteria1.pro1.iferation.1.ft56ttiqhyIMf1.8e忸+ria】CahKth.measurementforthenanop1.ate,squa1.ityandthesubsequentFurther,afternanop1.atequenchingthequorumsensingsigna1.,itcontinuestoadhereontobacteria1.浙曲号椰小殁耻耨防拙淞曲F嫄IkWa1.1.sen艘沁奥旧四弘U1.ar1.曲Rjhty盟涮困*榔dingonewiththepositive1.ychargedcationicsurfactant,isapp1.icab1.eforb1.ockingbacteria1.surfaceforpresumab1.yinhibitingthequorumsensing.Therefore,theovera1.1.antimicrobia1.efficacyiscorre1.ated制中曲M三w哨fe1.W1.entb留Ufi1.浙(喉H蝌A'<gs1.三ech解fiEBofChemicatmoietiesinc1.udingcarboxy1.,amide,phosphate,hydroxy1.andcarbohydratere1.atedmoieties,17imp1.yingthestrongaffinityfornanop)aw.Basedontheexperimenta1.data,theMBCva1.ueft8三1.W*ft三Xnn4正豳A分附b6VM帕E8aydrOPRM柚必酬IHbHi愧州ereMionicfo1.ddifferenceintheconcentrationrequiredtoinhibitbacteria1.growthbetweennegative1.ychargedc1.aynanop1.ateandmodifiedc1.aynanop1.ate.ThisphenomenonhasconfirmedtheconceptofQQ加藤晡叫跖t.can丽啼ia1.施鼎融跳磁期骗!fe11柘S4V三浏小R曲骼!,觎QQAispragmatica1.1.yusedtodea1.Withthepathogenicbacteriaandabroadspectrumofthebacteria1.co1.oniesinagricu1.turefie1.dsandsoi1.s.AnnexC(informative)Safetyofc1.aynanop1.atesC.1.Natura1.dayBentonitedayhasbeenontheFDA'sGRAS1.ist(Genera1.1.yRecognizedasSafe)formanyyears(CFR-Nactebfd(K1.-1.7Ubt(on.<rit1.eBimari1.y21)CfiR>i8ht>t8BifcqfMMiiiceJh>Biniodnjtf(cAfiMj11iRggistryquantitiesofiron,a1.ka1.iesanda1.ka1.ineearthsinthecommercia1.products.C.2C1.ayIianop1.ateThecytotoxicityandgenotoxicityofthec1.aynanop1.atederivedfromtheexfo1.iatedMMTwasstudiedbyDNA此m*geTu2HorhfQUndi1.让UihAherMihIJbtyaSmt中1.ate66ih<用I网作烛rin&VaryIgHOTce1.HaEPtbXicity.Themicronuc1.eustestindicatednosignificantchromosoma1.damageonmicefordosesupto500mgkgbodyweightandtheSa1.mone1.1.agenemutationassayonstrainsTA98,TA100,TA102,TA1535,and独药缸把独曼般dim制&由azo1.2出艺部邺林砌岫¾三fi如概佃tyaM1.1.&SW决购戚&能莫re1.easefromMTTand1.ayereddoub1.ehydroxides,respective1.y,showing1.owcytotoxicityonCHOce1.1.sbe1.ow1000gm1.after121.incubationperiodandadose-dependenteffectafter24hincubation.For国蜩心口用舟丧帆建幅心崂细'rtarcj酬狎的驰忸占渺工弗弗Ig叫超dJtiMca1.iterature.Maisanabaeta1.20observedthevarietyofthetoxicityconc1.usionsdescribedinthescientific1.iterature,theauthorfnundthatunmodifieddaynannp1.atesingenera1.inducedCytotOXiCitydependentHft而KedciayCO嵌gM6蹄为弗哈漉£你A&.experimenta1.system.MostquaternaryammoniumMostOfrtreTyreVioUSstudiesinrodentstreatedwithunmodifiedMMTshowednoc1.earevidenceof砥取M劭1曲曲州屋VaIUa1.%M鄢P瞅IM郑洲PnanO孤困丽麟喇VUrepor1.M9t1.ong.termdtaryexposuretoMMTat2,0%inSprague-Daw1.eyratsfor28wdidnotshowthepotentia1.toxicity.(2211.iaoeta1.observedthatdaynanop1.ateshowedhighbindingcapacityformycotoxinssuchasforW)三OP1.atediStUrbSh肌叫OUSe勖H三I碘VeI(Wa*%ha6h%bdPortt<Wr三medtothedietata1.eve1.of0,5%andfedtothepregnantratthroughoutpregnancy,theMMTa1.oneWaSnottoxicandcantight1.ybindaf1.atoxininthegastrointestina1.tractofanima1.s.(Z4Amodifiedwithin®位的幌4N*同点RK希隔吊弘由HJ嘛仃霸uf81.削!由我概&IOfi1.gpsh仲I假碗局呼曲y赳另VCnbeneficia1.toreducethetoxicityofFBIandzear1.enoneinSprague-Daw1.eyrats.C.3Effectofdaynanop1.ateonaqueousorganismsDrinkingwatersafetyhasbeenthreatenedbyincreasingharmfu1.a1.ga1.b1.oomsinwa1.ersources.Atthedndagea<Hdb1.M>o闺I甲m,th的inypfatkavystm1.RenHanoi(S(iK)琉叩qtAqRpp11h4i11peidedMicrwc1.tjsaeruginosa,usedintoxicitytestsperformedwithhazardousenvironmenta1.contaminantsre1.easedfromwateror福is阴刑除ion黜趣曲林阳相E¥BfNSrf1.1.rKItHfi0C幽加注联眠购AmcEifi概itutcmmf煦出慌cntbyISOoftheproductnamed.Equiva1.entproductsmaybeusediftheycanbeShoWntO1.eadtothesameresu1.ts.10Dm1.tOKtgU1.X<n1.exhdiitedobs11eryd6hatortfcday1.nydpUtedSPaParimWUhnaSCdUdk1.)it4.k浑IfttaumiMMbiS1.reportedthattheMMTcanprotectHydravu1.garisagainsta30ppmg1.yphosateand20ppmparaquatat04%,2Theya1.soreportedthatanacid-processedMMTat2%wassafeforHydravu1.garis,itcanprotectHydravu1.garisranging60%to70%againsta2ppmtoxicmixtureofPeStiCideS网Bib1.iography1P期即f09uaM欣熟fi1.1.喇fes6mesC1.deQM½)WMsK0WhaWnSOaM.,Springer,and2O1.Hea1.th(2Scho1.IenbergerGJ.,SimonR.H.,Soi1.Science,59(1),13-24(1945)(3)¾cte次Gcs2祥微曲前kigi*ftirednanomateriakPart1:Specificationof4ISO9277,Determinationofthespecificsurfaceareaofso1.idsbygasadsorptionBETmethod51通州&舷d%NAwSqbc/加面阴金碗Kn(ItEn巾f)Rm8而讪侬capacity(CEC)and6ISO22412,Partic1.esizeana1.ysisDynamic1.ightscattering(D1.S)7 ISO13320:2020,Partic1.esizeana1.ysis1.aserdiffractionmethods8 IS0(1499-2:2012,Co1.1.oida1.systemsMethodsforzeta-potentia1.determinationPart2:Optica1.211aC1.ayExfo1.iationandTheirUniqueSe1.f-Assemb1.yintoFibrousArrays",J.Phys.Chem.B2006r110.181110 WeiJ.C.rWangT.T.,1.inJ.JFirstEvidenceofSing1.etOxygenSpeciesMechanisminSi1.icateC1.ayforAntimicrobia1.Behaviour'*,App1.C1.aySci.992014,18-2311 WeiJ.C.rYenY.T.,SUH.1.rIJn.J.miInhibitionOfBacteriaIGrowthbytheExfo1.iatedC1.aysandObservationofPhysica1.CapturingMechanism",J.Phys.Chem.C2011,115,1877012 ThengBKG.TheChemistryofC1.ay-organicReactions.NewYork：ohnWi1.ey&Sons,197413 ChiuC.W.rChengW.Tv1.in).n,Exfo1.iationofsmectitec1.aysbybranchedpo1.yaminesconsistingofmu1.tip1.eionicsites*,EuropeanPo1.ymerJourna1.Vo1.44,1SSUe3,March2008,62863614 HsuS.h.,TsengH.JmHungH.S.,WangM.C,HungC.H.1.iP.R.,1.inJ.J.m./Xntimicrobia1.ActivitiesandCe1.1.u1.arResponsestoNatura1.Si1.icateC1.aysandDerivativesModifiedbyCationicA1.ky1.amincSa!tsw.App1.Mater.Interfaces2009r1,11,2556-256415 NaikS.P.rScho1.inJ.rChingS.,ChiF.,HerpferM.fttQuorumSensingDisruptioninVibrioharveyiBacteriabyC1.ayMateria1.s",J.Agric.FoodChem.2018,66,40-4416 阴Uka9mjZ4曲收4D.Wa1.kerS.rTheBacteria1.Ce1.1.Enve1.ope'；Co1.dSpringHarb.Pcrspect.1718JiangW.1SaxenaA.,SongB.,WardB.B.,BeveridgeT.J.,MyneniS.C.B.*,1E1.ucidation三,!R礼)S1.¾yP.4CgatiVCBaC1.eriaISiIrfaCCSUsinght1.ps：/www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCFR/CFRSearch.cfm?fr=184.115519 Id品Bt秘包f硒iUNnoc1.fiHaM邸陶雕r.E陶3加施陋d包好OtOXiCiIyand3)Underpreparation.Stageatthetimeofpub1.ication：ISO/DIS22171:2023.12202D222324252627282913031132H)343536WIMka)IOhiuS1.tfhmt1.o6pPd。翼Cnk1.C1.favodfiartiram*Br1.cfiA)rmeA)ME)wirArtnentaiResearch1382015r233-254微喧懒就.蝴EM部CteM献eMin怫缴维鹦蜕臆魏燃楹三赎那搬蝉Tana鼬搬Y蝌,随雷触螺脚嘴嘘WM崛略W蜃MUMhe牖。鹏0砺触脚U此棚揶ed服品解&砧秋阳腺把'阴鼠T。Xi部橘四福艇1.199-204BentOniteOnE1.-NekeetyA.A.,E1.-KadyA.A.,Abde1.-WahhabK.GvHassanN.S.,Abde1.-WahhabM.A二窿腑演3四空曲indivi三1.郴仙的地解力瞅®8能fumonisF粒EnVH砒Zear私岛撷翅比ie3iy724:20770-20783dRngmicEb£ystin.GH.,uV/?3也履即“卜用毁A*华摘S隰ph22remg1.4H(涵>Microcystisae11ginosaKryuchkovaM.,Dani1.ushkinaA.r1.vovY.rPakhrii1.1.inR.*,Eva1.uationoftoxicityofnanoc1.aysand452grapheneoxideinvivo:aParameciumcaudatumstudy*,Environ.Sci.:Nano,2016,3,442-WangM.,OrrA.A.,HeS.,Da1.aijamtsC.,ChiuW.A.,TamamisP.,Phi1.1.ipsT.D.*,rSfWnMaR阉1帝邮andParaquatReducingToxinExposuresandWangM.,Phi1.1.1.pT.D.nrPotentia1.App1.icationsofC1.ay-BascdTherapyfortheReductionofPesticideExposuresinHumansandAnima1.s*,App1.Sci.2019r9,5325ISO/TS80004-2:2015,Nanotechno1.ogiesVocabu1.aryPart2:Nano-objects1SOTS80004-4:2011,Nanotechno1.ogiesVocabu1.aryPart4:Nanostructuredmateria1.s科区mgffq?KnJWW照龙自丽近而g/a皿砒"以用册制#g<3¼v麻z/涮碓(W明忖H1.fS柄92Co1.1.oida1.systemsMethodsforzeta-potentia1.determinationPart2:Optica1.惬疔帖W,5三pfj恸阕腑济欣的居阴也WM4/”心Egtheinvitroactivityofantimicrobia1.agentsagainstrapid1.ygrowingaerooicbacteriainvo1.vedininfectiousdiseases4C51fW#0111MethodsforDi1.utionAntimicrobia1.Susceptibi1.ityTestsforBacteriaThQtGrow6tS1.(-,MethodsforDeterminingBactericida1.ActivityofAntimicrobia1.Agents；Approved