First report of leaf spot on Angelica japonica caused by Septoria aegopodina in China.docx

《First report of leaf spot on Angelica japonica caused by Septoria aegopodina in China.docx》由会员分享，可在线阅读，更多相关《First report of leaf spot on Angelica japonica caused by Septoria aegopodina in China.docx（2页珍藏版）》请在课桌文档上搜索。

1、Firstreportof1.eafspotonAnge1.icajaponicacausedbySeptoriaaegopodinainChina4 H.J.Xu.R.J.Zhou.1.EZhao.1.EFu.andN.HaoDepartmentofP1.antPatho1.ogy.Co1.1.egeofP1.ant5Protection.ShenyangAgricu1.tura1.University.Shenyang.1.iaoning.110866.China67 Ange1.icajaponicaA.Gray.aperennia1.herbintheUmbe1.1.a1.esfami

2、1.y,iswide1.ydistributedinChina8 asamedicina1.herbandvegetab1.e.InAUgUS1.2015,IeafSPOtsymptomswereobservedon1.eavesof9 A.japonicainther(x1.uctionI1.e1.dsofDengchangvi1.1.age,Xinbin,1.iaoning,China.Thedisease10 main1.yaffectedthe1.eavesandstems,withsymptomsoccurringonapproximate1.y25to50%ofthe11 p1.a

3、ntsreducingyie1.dbyabout30%.Initia1.1.y,symptomsdeve1.opedonbothsidesof1.eavesas12 scatteredpinpoint1.ightbrownspots.As(he1.esionsexpanded,theydeve1.opedintopa1.ebrown,13 e1.1.iptica1.orirregu1.ar1.yshaped1.esionsthateventua1.1.ybecamedryandgrayishorwhiteinco1.or14 bearingpycnidiasurroundedbybrownvi

4、o1.etnecroticmargins.Numerous1.esionsbecamecracked15 atthecentersandcoa1.escedtogether.CondiaIbnnedinIhepycnidiaweret1.1.ifo11nto16 fi1.iform-cy1.indrica1.,Stniightorcurved,gradua1.1.yattenuatedtoanarrow1.yroundedtopointedapex,17 andInOrCor1.essattenuatedtowardsthenarrow1.ytruncatebase.Theywere(0)1.

5、-3-scptate.hya1.ine.18 withsevera1.oi1.-drop1.eisandminutegranu1.armateria1.ineachce1.1.,21.4to42.71.4to2.5rn19 (average26.52.1m),Thepathogenwasiso1.atedfromsymptomatictissues,andcu1.turedon20 PDAat25for2to3wk.Theco1.onywasrestrictedandSOmCwhate1.evated,producingdense.21 whitetograyaeria1.myce1.iumt

6、hat1.aterbecamedarkerwithanaveragegrowthrateof0.35nn11day.22 After20d.s1.imy,sa1.mon-co1.oredconidia1.masseswereinterspersedwithintheco1.onics.Sequences23 ofsix1.oci,trans1.atione1.ongationfactorI-a1.pha(tcfI).beta-tubu1.in(TUB),RNApo1.ymeraseI1.24 second(R1,B2).1.argestsubunit1.argesubunitribosoma1

7、.(1.SU),interna1.transcribedspacer(ITS),25 andca1.modu1.in(CA1.),wereamp1.ifiedwithprimersEFI-728Fef-2,T1-Sandy-R,26 fRPB2-5FfRPB2-41.4R.1.SUIFd,1.R5,ITS5ITS4,andCA1.-235FCA1.2Rd.respective1.y,and27 sequenced(Quaedv1.iegeta1.2013;Verk1.eyeta1.2013).Thephy1.ogenetictreewasproducedusing28 MECA4.0,andn

8、eighbor-joiningana1.yseswereerfonnedusingthecombineddatasetsofthesix29 regions,formedasing1.ec1.adewithmaximumsupport(100%)withsequencesofSeptoria3()aego)odinaSacc.Iheresu1.tingsequences,tef1.(550bp).TUB(480bp).RPB2(367bp).1.SU(88631bp),ITS(507bp),andCA1.(422bp)weredepositedinGenBankasKU921437toKU92

9、I453and32 KT985904.respective1.y.Thus,thefunguswasidentifiedasS.aegopdinaonthebasisof33 morpho1.ogica1.andcu1.tura1.characteristics(Verk1.eyeta1.2013)andmu1.ti-1.ocusphy1.ogeneticana1.ysis34 (Verideyeta1.2013).Toconfinnthepathogenicity,fivedetached1.eavesofA.japonicawere35 inocu1.atedwithaConidia1.s

10、uspension(IO6conidiam1.1).Contro1.1.eavesweretreatedwithsteri1.e36 disti1.1.edwateron1.y.The1.eavesweremaintainedinap1.asticcontainerinthegreenhouse(re1.ative37 humidityabout95%)at25innatura1.day1.ightandobservedperixiicaHy.Symptomson1.eaves,38 identica1.Iothoseobservedinthefie1.ds,werevisib1.e8toIO

11、daysafterinocu1.ation.No1.esions39 deve1.opedonthecontro1.1.eaves.Thesamefunguswasre-iso1.atedfromthesymptomatictissue.40 identifiedbymorpho1.ogy,comp1.etingKochspostu1.ates.oourknow1.edge,thisisthefirstforma1.41 reportof1.eafspotonA.japonicacausedbyS.aegpdinain1.iaoning,Chinaanditmaybeusefu1.42 intheestab1.ishmentofabaseforscientificCon1.rO1.ofthedisease.Theinfo11nationdescribedhere43 mayhe1.pgrowersmanagethedisease.4445 Keferences:46 W.Quacdv1.icgcta1.StudMyco1.75:307,2013.47 GJ.M.Vcrk1.cycta1.StudMyco1.75:213,2013.