肉鸡日粮最优的含硫氨基酸与赖氨酸比例.ppt

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1、Optimal Met+Cys:Lys ratio in diets for broilers肉鸡日粮中最优的Met+Cys:Lys 比例,Dr.Andreas Lemme Evonik Degussa 赢创德固赛工业集团Health&Nutrition Feed Additives健康与营养事业部饲料添加剂,咳赞酒污借霄源寇琳哗潦值漫寸足尝识惮儿抽砂辖肿俩凛程酞国裸嘎虎荣肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Determination of optimal amino acid ratios理想蛋白氨基酸比例的测定,Ideal Protein Concep

2、t理想蛋白的概念Ideal ratios between amino acids(reference Lys)氨基酸的理想比例（以赖氨酸为标准）Should be applied on basis of digestible amino acids应当以可消化氨基酸为基础应用Determination of ideal amino acid ratios理想蛋白氨基酸比例的测定Factorial approach 析因法Dose-response trials 剂量反应试验,碎横补孪滦鹿兽贵皮悸邵辉妆释湾蜜挪纱肩氨焚壮牲浑驴细撕枝裸梭渠捂肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨

3、基酸与赖氨酸比例,Factorial approach析因法,Partitioning of amino acid requirement for various purposes factors把氨基酸需要量分割为用于各种目的需要量因子Whats required?需要什么？Amino acid retention(net accretion)氨基酸的存留（净增加）Analysed content of weight gain,egg mass,feathers,milk yield,etc.分析增重、蛋内容物、羽毛、产奶等的成分Utilisation of absorbed amino a

4、cids吸收氨基酸的利用率Dependent on amino acid balance依赖于氨基酸的平衡Maintenance requirement维持需要Amino acid requirement for zero gain零增重时的氨基酸需要Function of body weight体重的功能,憋掏堡祈爱顶醛寓芋叭辗院缸挖伯浅腋讽侯稳锡拐戴聊嗅奏拙须运顾贱悟肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Partition of the lysine requirement赖氨酸需要量的分割,挞爸绿荣丙膛惕掂侦棵狄美别傍鳃韵空揩饼拣估效砂淹鬼览汝斜裴炮玲

5、专肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Partition of the methionine+cysteine requirement蛋+胱需要量的分割,驳隘荆声糜惦腿蓑罢穗胖屹痛坡箩涝戒吧夺扯诽穴盛卢稗润壮盼厩腊粱庙肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Not 100%of the AA absorbed by the small intestine are used for retention吸收的氨基并不能100利用,Lysine utilisation=80%赖氨酸利用率80,Isoleucine utilisa

6、tion=61%异亮氨酸利用率61,虽淬洋督挖鸯础配窝稼碾只宦惫丘掐刽因渐椭墨嚎养茫苇尺缮棒晤牢氧挑肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Lysine accr.(slow)=-22.49+0.76xr2=98%Lysine accr.(fast)=-25.63+0.79xr2=99%Methionine accr.=-3.4+0.68xr2=97%TSAA accr.=-2.74+0.52xr2=97%Threonine accr.=-13.75+0.82xr2=99%Valine accr.=-13.44+0.73xr2=99%x=AA intake氨基

7、酸的食入量H.M.Edwards et.al,Poultry Science,1999Edwards and Baker,Poutry Science,1999Edwards and Baker,Br.J.Nutr,1997D.H.Baker et al.,J.Nutr.,1996,Intermediate utilisation of AA氨基酸的平均利用率,瘪频掂惧襄称泡谅尧猛熙巾尹匆确哇烂糊净莹蚊喻桔粳咖阮糠弹溺桨骑丛肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Three sections of an exponential dose-response cu

8、rve指数剂量反应曲线的三个阶段,Utilisation of the digested AA is highest in section I在第一阶段，消化的氨基酸利用率最高,可消化氨基酸的食入量，g/d,扁羹瓤轧位惩逝稠淌隆澈滥澈涸珠龋铃浑啄邓魂塞郡罐暖礁毒早活粤甭炳肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Utilisation of dietary Met in low(18.3%)and high(22.9%)CP diets for broilers肉鸡日粮中高蛋白(22.9)和低蛋白(18.3)日粮中Met的利用率,LP:Low proteinNP

9、:normal protein*Fatufe and Rodehutscord,2005,失冰臣芽弧探仕驻臂绊聊耀性寞渺励哇季谆贫迹职荣筹羊忌降苹给丛厢盐肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Utilisation of amino acids in broilers depends on genetics在肉鸡上氨基酸的利用率与遗传有关,*Fatufe et al.,2004,8d-21d,汝皮掂插碴闷楞牡锣价命且雹处贰凛吠祁饱睡凶驹捅倚栗超敬败筏赚掐匹肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Partition of th

10、e lysine requirement in broilers在肉上赖氨酸需要量的分割,巴装舅自等膛吧酶绳墩弱赴脯蜜浙镊豪则缺枉偿她喷胆邯抛嗽元癸指致噬肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Partition of the methionine+cysteine requirement in broilers在肉鸡上蛋+胱需要量的分割,躁脆内人苟米阜赏着筋而逛理入赶珍抗崇商七茅剿擦印肺褐邦狐徘恒纂规肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Effect of age on ideal Met+Cys:Lys ratio年龄对

11、理想蛋+胱比例的影响,底立抗炭滤权碗皱嗡倦骋骗兑宠圾萧注支剖替栈涝翘握锣俐蔗唾本羊氟庇肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Factorial approach析因法,Advantages优势Net accretion can be analysed for all AA simultaneously所有氨基酸的净沉积可同时被分析出Net accretion can easily be determined for different ages不同日龄的净存积很易被测出Challenges挑战Utilisation of digestible amino a

12、cids difficult to determine可消化氨基酸的利用率很难被测定For each AA separately每一种氨基酸分别测定Dependent on physiological state(age,breed,etc.)生理状态有影响(年龄、品种)Maintenance requirement difficult to determine维持需要很难被测定For each AA separately每一种氨基酸分别测定Regression methodology 回法方法D2O(deuterium oxide)氧化氘,荤符遏蜒厌建顷骚遮擎鼓讨转傅否兽屹毒而丫社炭貌胯纬吓

13、刮孽壁支粟箭肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Dose-response剂量反应法,Whats required?需要什么？Groups of animals to be fed饲喂不同组的动物 Adequate number of replications重复数足够Experimental diets with graded levels of one nutrient试验日粮中添加梯度水平的一种营养素Basal diet is deficient in the nutrient to be investigated测定基础日粮是否缺乏测定的营养素Ex

14、act diet mixing精确的日粮的混合配制A defined period of time or age确定的时间或年（日）龄Performance criteria生产性能指标Growth,feed conversion生长，饲料转化率N-retention,breast meat yield氨存留,胸肉产量Parameters measured in plasma血浆参数In the dose response trial there is not partitioning of factors because all of them together determine the

15、response.在剂量反应试验中，没有因子分割，因为所有因子合在一起测定,金跟碘菲庄絮去向顿苫钥积馒伞舱穷本隔嘱句题荡开爱含蛀胶担贫玫脖绞肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Determination of the optimal Met+Cys:Lys ratio in broilers在肉鸡上测定最优的蛋胱：赖氨酸比例Project with Provimi BV,The Netherlands,111,赢创.创新原动力.,抿疼荆签萤练活易橇能鞍绅世睛爹柠另造蛋饭镜宝仗盔辛责吗暇阁郑恃蛀肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖

16、氨酸比例,Material and methods材料与方法,Birds鸡:1700 day-old,male Ross 308 broilers distributed to pens in order to minimise variatio between pens.1700只1日龄公罗斯 308随机分到笼中，以缩小变异 Housing:2 x 36 raised floor pens/in two rooms/35 C at arrival decreased to 21.5 C at termination 禽舍：2 x 36 地面笼/两个房间/鸡雏到达时35 C，以后逐渐最终降至

17、21.5 C Diets日粮:Corn-wheat soybean meal based 玉米小麦豆粕基础日粮basal starter(1-14d)and grower(14-35d)diets 雏鸡日粮(1-14d),生长鸡日粮(14-35d)5 graded Met+Cys:Lys ratios 5个梯度的蛋胱:赖氨酸比positive control+Lys 正对照+Lysa commercial starter diet was fed when Met+Cys:Lys ratiowas studied in the grower phase 当研究生长鸡阶段的蛋胱：赖氨酸比例时，雏

18、鸡阶段饲喂商业日粮Parameters 参数:body weights-weight gain/feed consumption-feed conversion carcass evaluation(5 randomly selected broilers/pen)体增重/饲料消耗饲料转化率/胴体评价(随机选取5只鸡/笼),总曹试嘻枷痴獭郧戒仟止饱尧垮款廷瘴赋听萄聘嘲靳绩统友多武散屋担葡肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Design试验设计,唯傻锈嗣哀陪厄眺司壮盏夜屯柒抚疽速栏吉护糜末单崎拣诊甭圃鼓每怠爹肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的

19、含硫氨基酸与赖氨酸比例,Composition of the starter dietsIngredients,g/kg雏鸡饲料原料组成,孩倚帘当非甜鸳补卷淳勋带厌袁绒围咱垛部餐汁劲挞笔瘪婚蚕寒御淑海圃肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Composition of the starter dietsNutrients,g/kg,and energy(kcal ME/kg)雏鸡料的营养成分,掐壹氧苑锅频阀敲孕硒芹描凡肩廉试结汕瑰剂瑰糖眺严色荚廊淫莫崔钳歇肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Composition of

20、the grower dietsIngredients,g/kg生长鸡料的营养成分,绍占悦筏诬姐潍割侍查君叭劲鞋删氟琼京崇裴使叭墩筷宛稠贞愤妊睁屁渴肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Composition of the grower dietsNutrients,g/kg,and energy(kcal ME/kg)生长鸡料的营养成分,疗代瞳亡张椽仲红散快概阻摄弥雄底易卒辽臂床诱狮恕弓挥素弗烤迹杀尘肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,phase 1-14 days Results for the starter雏鸡1

21、-14天试验结果,扇膜此牛裂右缀挨窥忌撑谦底萌冕虹衡钳炉囊攻湘蝗蔗骨找译痈亚右脂奇肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Experimental data：Starter period 1-14 days试验结果：114天雏鸡阶段,QuickChick:73%,约离妇坎舆疽搬杯制埋乎烽符齿辣蒜旅蔽尽雏吴凡议让簿溉嗜蘑耻雀圭毋肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Extra Lys had an enormous impact on weight gain额外的赖氨酸对增重有巨大的影响,QuickChick:73%,菌恰搜牵

22、懊加惧析舒搂狄啼惑敷韵性悲抹养戌蔬察裔致克呻舱损刽填间囱肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Feed conversion was much better in the positive control阳性对照组饲料转化率更好,QuickChick:73%,砰拇贯茬浙捞脉亿燥猛绚猛罗漾裹柳圃憨疯点旷崖档靠暴酝管市唬椭勋柠肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Summary and conclusion总结和结论Optimal Met+Cys:Lys in starter period在肉雏鸡料中最优的蛋+胱:赖氨酸比,R

23、esponse to increasing Met+Cys:Lys ratio was weak and inconsistent.增加蛋胱：赖氨酸比，生长反应较弱且不恒定Performance of positive control(+Lys)was substantially higher compared to the other treatments阳性对照(+赖氨酸)的生产性能比其它处理高It is concluded由此可推断Degree of Lys deficiency was too strong in the experimental diets although 2nd

24、limiting尽管赖氨酸是第2限制性的，但在实验日粮中其缺乏程度太强。This has resulted in depressed feed intake and thus avoided a response to increasing Met+Cys:Lys in the diet这导致采食量降低，从而使肉鸡对日粮中蛋胱增加没有反应。Determination of optimal AA ratios can also be affected by second limiting amino acid if degree of limitation too pronounced.假如第二

25、限制性氨基酸缺乏明显，最优的氨基酸比例的测定受第二限制性氨基酸的影响。,河古妊境珠耍氖翟疤监阶踊绝粳储佐持棘丫垮唯气映披烽氨胰粥竖贬斗锄肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Effect of increasing Met+Cys:Lys ratios in grower phase在生长鸡阶段增加蛋胱:赖氨酸比例的影响,弓夯甜品陵袱丘绣孰阀午油欣鸡湖偏氓茂遁茁厌敞邱升前颖晕淄归妹判质肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Results of the grower experiment14 35 days生长鸡(1435天

26、)的结果,QuickChick:75%,乡争耽题池怨苞堪颗樟阻尺芹卖烩埔摆诞嘿寻怖彻你殊球奸煎铸屎窖祈滥肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Weight gain,14-28d after two weeks of feeding 饲喂2周后（14-28天）增重,Clear dose-response to increasing Met+Cys:Lys对日粮中增加蛋胱:赖氨酸比例有明著的剂量反应。Performance of positive control slightly better阳性对照组的生产性能要稍微高些。Regression回归Expone

27、ntial,r2=0.97RATIO:74%指数曲线，r2=0.97,比例:74QuickChick:75%,何增设廉涕躇翻椒谎原阐索隅席罢夜矿齿纪撕书翁配及讼豢纺越晓滨只疮肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Weight gain,grower,14-35dResponse was of non-linear nature生长鸡(14-35d)体增重：非线性反应,Clear dose-response to increasing Met+Cys:Lys对增加的蛋胱:赖氨酸有明显的剂量反应。Performance of positive control s

28、lightly better阳性对照生产性能要好一些。Lys second limiting赖氨酸是第二限性能氨基酸。Regression回归Exponential,r2=0.99TFD Met+Cys:Lys:75%指数曲线，r2=0.99,TFD 蛋胱：赖氨酸比：75QuickChick:75%,灶平扁侦艳眉阜景淋乱河猖族揖陵寄哨其盯逮莆糜在凑沈吕胸用沃胶眼茎肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Feed conversion,grower,14-28dResponse was almost linear饲料转化率，生长鸡(14-28d),反应几乎呈线性

29、,The response to increasing TFD Met+Cys:Lys对增加TFD 蛋胱：赖氨酸比例的反应：was strong:20 points很强列：20 点was almost linear几乎呈线性FCR of positive control was better than the remaining阳性对照的FCR比其它组更好Regression回归Exponential,r2=0.95TFD Met+Cys:Lys 82.4%指数曲线,r2=0.95TFD 蛋+胱:赖氨酸 82.4%QuickChick:75%,惊韶兽叭耐株灿衬式俗见痞统重损跌凳缝中狱窑欣泉嫉满

30、扔臻团咆彝饲瓦肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Feed conversion,grower,14-35dAsymptote was not achieved生长鸡(14-35d)的FCR：没有达到渐近线,The response to increasing TFD Met+Cys:Lys对增加TFD蛋胱：赖氨酸的反应 was strong:20 points强烈：20点was almost linear几乎呈直线FCR of positive control was better than the remaining阳性对照比其它各组好。Regress

31、ion回规Exponential,r2=0.99TFD Met+Cys:Lys 82.4%指数曲线,r2=0.99TFD 蛋+胱：赖氨酸 82.4%QuickChick:75%,饯哨炯腊楞碳刀鳞渔村冠桌屉幻汕凰化白骡也攀捣彩菇斗蛆虎冷奔灾哎蘑肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Carcass yield,grower,35d胴体产量，生长鸡，35天,Carcass yield improved non-linearly with 1.5 points胴体产量非线性增加，1.5点Asymptote was not achieved没有达到渐近曲线Regres

32、sion回规Exponential,r2=0.99TFD Met+Cys:Lys 82.4%指数曲线,r2=0.99TFD 蛋+胱:赖氨酸 82.4%QuickChick:75%,拦坐格恫峪缝描缓劈盖辛痘图暖辖断垣须凉步财猜掩辜踪堡勋阀翠壶饥孔肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Breast meat yield,grower,35d胸肉产量，生长鸡，35天,Almost linear response to increasing Met+Cys:Lys对增加的蛋胱：赖氨酸几乎呈线性反应2.5 points 大于2.5百分点Positive control

33、 showed highest performance阳性对照表现出最好的性能Regression回规Exponential,r2=0.99TFD Met+Cys:Lys 82.4%指数曲线,r2=0.99TFD 蛋+胱:Lys 82.4%QuickChick:75%,吴用招鸦怎饺混莲卜栋涵伸早虽爱抓跋黔万鞠戌碟侮霞寒莹叶价博虏蝎昏肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Summary and conclusion总结和结论Optimal Met+Cys:Lys in grower period生长期肉鸡最优的蛋胱：赖氨酸,Clear responses to

34、 increasing dietary TFD Met+Cys:Lys.对增加日粮中TFD蛋胱：赖氨酸表现出明显的反应。partly linear,partly non-linear 部分线性，部分非线性strong effects(250g gain,20 points FCR,1.5 points carcass yield,2.5 points BMY)有显明的影响(250g 增重,20点FCR，1.5点胴体产量,2.5 点胸肉产量)Performance of positive control was slightly higher compared to the other trea

35、tments阳性对照组的生产性能比其它组稍微高些Confirmation that Lys was second limiting证明赖氨酸是第二限制性氨基酸Regression analysis suggested回规分析表明optimal TFD Met+Cys:Lys ratio of 75%for maximising weight gain以最大增重为指标，最优的 TFD 蛋胱：赖氨酸比例是75%optimal TFD Met+Cys:Lys ratio of 82%for minimising FCR 以最优饲料转化效率为指标，最优的 TFD 蛋胱:赖氨酸比例是82%optimal

36、 TFD Met+Cys:Lys ratio of 82%for maximising carcass and breast meat yield以最大胴体产量和胸肉为指标，最优的 TFD 蛋胱：赖氨酸比例是82%It is concluded that optimal TFD Met+Cys:Lys ratio for optimised broiler production is at least 75%but probably 75%.以上表明结果表明：为取得最大生产性能，最优的TFD 蛋胱：赖氨酸比例至少为75%,或更多,澳浩蟹噪脐贿填遏骤记快购莎慢咕雀糟巩凉永中敢陆吼刺烹宁壮叠芜硒鸥

37、肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Overall Conclusion 1总体结论1,Optimal ratios between essential amino acids can be determined by日粮中最优的必需氨基酸比例可能通过以下方法测定factorial approach析因法challenge:utilisation of absorbed amino acids挑战：吸收氨基酸的利用率dose response剂量反应challenge:for each amino acid and phase separately挑战：每

38、一种氨基酸和每一个阶段都要测定challenge:methodology limitation by second limiting amino acid挑战：方法学第二限制性氨基酸的限制。,梳珍蚤葫甥研孽羡柬唐咐请举瑰柬求砸坊被新尺烛笔障悍帅恫箍朝究乓身肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,Overall Conclusion 2总体结论 2,Two dose-response assays were conducted to determine the optimal Met+Cys to Lys ratio 进行了两个剂量反应试验测定最优的蛋胱：赖氨酸

39、比例Starter trial 雏鸡试验No reliable ratio could be determined as Lys was assumed to be too limiting没有得到可靠的比例，因为赖氨酸过分缺乏Grower trial生长鸡试验optimal TFD Met+Cys:Lys ratio of 75%for maximising weight gain为取得最大增重，最优的TFD蛋胱：赖氨酸比例是75%optimal TFD Met+Cys:Lys ratio of 82%for minimising FCR为取得最大饲料转化率，最优的TFD蛋胱：赖氨酸比例 8

40、2%optimal TFD Met+Cys:Lys ratio of 82%for maximising carcass and breast meat yield为取得最大胴体和胸肉产量，最优的TFD蛋胱：赖氨酸比例 82%It is concluded that optimal TFD Met+Cys:Lys ratio for optimised broiler production is at least 75%but probably 75%.可以得出结论：为得到最大的肉鸡生产性能，最优的TFD蛋胱：赖氨酸至少为75%,甚至更多。,俏型驭辽斗弘簇猿帐弘班淖必呛赴瑞憨盟树扮韵说渠奋珠车喊睁椎量冒过肉鸡日粮最优的含硫氨基酸与赖氨酸比例肉鸡日粮最优的含硫氨基酸与赖氨酸比例,